MCB - ITA

Revised July 2017

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your PowerPoint files can be converted to PDF without any errors. We strongly recommend that before returning their mod- ified manuscripts, authors check the acceptability of their digital images for production by running their files through Rapid Inspector, a tool provided at the following URL: http: //rapidinspector.cadmus.com/RapidInspector/zmw/index.jsp. Rapid Inspector is an easy-to-use, Web-based application that identifies file characteristics that may render the image unus- able for production. Please note when using Rapid Inspector to check PowerPoint files that there is a known bug in the appli- cation that can occasionally fail PowerPoint Presentation (.pptx) files, even though the files meet all required production criteria. If you experience this bug, the issue can be corrected by saving the PowerPoint files as an older version, PowerPoint 97-2004 Presentation (.ppt), during the Save As process (use the drop-down format menu and select this format). Once you save your files as .ppt, they will pass Rapid Inspector if all re- quired production criteria have been met. If you have additional questions about using the Rapid In- spector preflighting tool, please send an e-mail inquiry to helpdesk.digitalartsupport@cenveo.com. Minimum resolution. It is extremely important that a high enough file resolution is used. All separate images that you import into a figure file must be at the correct resolution before they are placed. (For instance, placing a 72-dpi image in a 300- dpi EPS file will not result in the placed image meeting the minimum requirements for file resolution.) Note, however, that the higher the resolution, the larger the file and the longer the upload time. Publication quality will not be improved by using a resolution higher than the minimum. Minimum reso- lutions are as follows: • 300 dpi for grayscale and color • 600 dpi for combination art (lettering and images) • 1,200 dpi for line art Size. All graphics should be submitted at their intended publication size; that is, the image uploaded should be 100% of its print dimensions so that no reduction or enlargement is necessary. Resolution must be at the required level at the sub- mitted size. Include only the significant portion of an illustra- tion. White space must be cropped from the image, and excess space between panel labels and the image must be eliminated. • Maximum figure width: 6.875 inches (ca. 17.4 cm) • Maximum figure height: 9.0625 inches (23.0 cm) Contrast. Illustrations must contain sufficient contrast to be viewed easily on a monitor or on the printed page. Labeling and assembly. All final lettering and labeling must be incorporated into the figures. On initial submission, illus- trations should be provided as PDF files, with the legends in the text file and with a legend beneath each image to assist review. At the modification stage, production-quality digital figure files (without legends) must be provided. Put the figure num- ber well outside the boundaries of the image itself. (Number- ing may need to be changed at the copyediting stage.) Each figure must be uploaded as a separate file, and any multipanel figures must be assembled into one file; i.e., rather than up- loading a separate file for each panel in a figure, assemble all panels in one piece and supply them as one file. Fonts. To avoid font problems, set all type in one of the following fonts: Arial, Helvetica, Times Roman, European PI, Mathematical PI, or Symbol. Courier may be used but should be limited to nucleotide or amino acid sequences, where a non- proportional (monospace) font is required. All fonts other than these must be converted to paths (or outlines) in the ap- plication with which they were created. Color illustrations. All figures submitted in color will be processed as color. Adherence to the following guidelines will help to ensure color reproduction that is as accurate as possible. The final online version is considered the version of record for MCB and all other ASM journals. To maximize online re- production, color illustrations should be supplied in the RGB color mode as either (i) RGB TIFF images with a resolution of at least 300 pixels per inch (raster files, consisting of pixels) or (ii) Illustrator-compatible EPS files with RGB color elements (vector files, consisting of lines, fonts, fills, and images). CMYK files are also accepted. Other than in color space, CMYK files must meet the same production criteria as RGB files. The RGB color space is the native color space of computer monitors and of most of the equipment and software used to capture scien- tific data, and it can display a wider range of colors (especially bright fluorescent hues) than the CMYK (cyan, magenta, yel- low, black) color space used by print devices that put ink (or toner) on paper. For reprints, ASM's print provider will auto- matically create CMYK versions of color illustrations from the supplied RGB versions. Color in the reprints may not match- that in the online journal of record because of the smaller range of colors capable of being reproduced by CMYK inks on a printing press. For additional information on RGB versus CMYK color, refer to the Cadmus digital art site, http://art .cadmus.com/da/guidelines_rgb.jsp. Drawings Submit graphs, charts, complicated chemical or mathematical formulas, diagrams, and other drawings as finished products not requiring additional artwork or typesetting. All elements, includ- ing letters, numbers, and symbols, must be easily readable, and both axes of a graph must be labeled. When creating line art, please use the following guidelines: (i) All art must be submitted at its intended publication size. For acceptable dimensions, see "Size" above. (ii) Avoid using screens (i.e., shading) in line art. It can be difficult and time-consuming to reproduce these images with- out moire´ patterns. Various pattern backgrounds are prefera- ble to screens, as long as the fill patterns are not imported from another application. If you must use images containing screens, Instructions to Authors July 2017, Instructions to Authors mcb.asm.org 15 Molecular and Cellular Biology

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